|
KMID : 0545120050150010131
|
|
Journal of Microbiology and Biotechnology
2005 Volume.15 No. 1 p.131 ~ p.135
|
|
|
Cell Age Optimization for Hydrogen Production Induced by Sulfur Deprivation Using a Green Alga Chlamydomonas reinhardtii UTEX 90
|
|
Kim JP
Kang CD/Sim SJ/Kim MS/Park TH/Lee DH/Kim DJ/Kim JH/Lee YK/Pak DW
|
|
|
Abstract
|
|
|
|
Under sulfur deprived conditions PS II and photosynthetic O2 evolution by Chlamydomonas reinhardtii UTEX 90 are inactivated resulting in shift from aerobic to anaerobic condition. This is followed by hydrogen production catalyzed by hydrogenase. We hypothesized that the photosynthetic capacity and the accumulation of endogenous substrates such as starch for hydrogen production might be different according to cell age. Accordingly we investigated (a) the relationships between hydrogen production induction time of sulfur deprivation increase of chlorophyll after sulfur deprivation and residual PS II activity and (b) the effect of initial cell density upon sulfur deprivation. The maximum production volume of hydrogen was 151 ml H2/l with 0.91 g/l of cell density in the late-exponential phase. We suggest that the effects of induction time and initial cell density at sulfur deprivation on hydrogen production up to an optimal concentration are due to an increase of chlorophyll under sulfur deprivation.
|
|
|
KEYWORD
|
|
|
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
  
|